Our Scientific Research - Experiment results - Daphniae

Death Ratio (%) 5 2 12
Comment: In Daphniae, two abnormal peaks of mortality can be observed (Zanolo, 1993). In both cases, the EMF-Bioshield® protection system ensured instead normal growth and development.

1.1  Mortality of the parental animals
All the individuals of the control group survived until the end of the study. The survival rate decreased to about 85% and 88% for the Daphniae exposed to VDT radiation without and with EMF-Bioshield®.

1.2  Day of the first brood
In the control group the first brood was found on day 9 of observation, while in both the other groups the first broods were found on day 7 of observation.

1.3  Live young
Considering all 21 days of observation, the total mean brood sizes of the animals exposed to VDT radiation both with and without EMF-Bioshield® were significantly lower than that of the control group. If considered in detail, the mean brood size of the Daphniae exposed to VDT equipped with EMF-Bioshield® was significantly lower (**p < 0.01) than that of the control group at days 14 and 16 of observation, while the mean number of live young was significantly higher (p** < 0.01) than that of the control group on the last day (21) of observation. On days 12 and 19 of observbation the mean brood size was not statistically different from that of the control group.
The Daphniae exposed to VDT without EMF-Bioshield® produced broods significantly lower in size than those of the controls on days 9, 14 and 16 (*p < 0.05) and day 12 of observation (**P < 0.01) than that of the control group.
When comparing the trend of the mean brood size vs. observation time curves, we see that the curve of the control group and that of the group exposed with EMF-Bioshield® can almost be superimposed, while that of the group exposed without EMF-Bioshield® has a completely different trend. The only similar points between the two groups exposed with and without EMF-Bioshield® are at the beginning of the brood production (day 7) and at the end of the study (days 19 and 21).

1.4  Dead young
The mean number of dead young found in both animal groups exposed to VDT with and without EMF-Bioshield® were always comparable to that of the control group except at day 12 and 14 observations when the mean number of dead young of the treated groups was significantly (and respectively) lower than that of the control group.

1.5  Non-hatched eggs
In the control group non-hatched eggs were not found at any water renewal, whereas in the group exposed to VDT without EMF-Bioshield® they appeared the day the Daphniae had the first brood, and in the group exposed to VDT with EMF-Bioshield® the non-hatched eggs appeared the day of the second brood, and were then found at each water renewal in both treated groups, the mean number varying from 0.08 to 0.25 in the group exposed to VDT withtou EMF-Bioshield® and from 0.06 to 0.21 in the group exposed to VDT with EMF-Bioshield®.

1.6  Parameter checking
The dissolved oxygen, the pH and temperature always remained within accepted limits.



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